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. 2022 Feb 8;12:820463. doi: 10.3389/fendo.2021.820463

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Copyright © 2022 Zhao, Chen, Liu, Jiang, Chen, Chen, Song, Tao, Cui, Li, Zhu, Chen, Hu and Luo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differentially expressed genes (DEGs) and functional analysis across testicular development from the first stage to the sixth stage. (A) Bar chart showing numbers of DEGs between consecutive time points in the brain and the testes. UpSet plots indicate numbers of the common and stage-specific up-regulated (B) and down-regulated (C) DEGs in the testis. (D) KEGG pathway enrichment map showing the top 5 significant pathways of upregulated and downregulated testis-specific DEGs for five stage-by-stage comparisons. Red font, green font, and blue font representing pathways enriched by upregulated DEGs, pathways enriched by downregulated DEGs, pathways enriched by upregulated and downregulated DEGs, respectively. Venn diagram representation of the number of common and stage-specific up-regulated (E) and down-regulated (F) DEGs in the brain. (G) KEGG pathway enrichment map showing the top 5 significant pathways of upregulated and downregulated brain-specific DEGs for three stage by stage comparisons during testis development. Red font, green font, and blue font representing pathways enriched by upregulated DEGs, pathways enriched by downregulated DEGs, pathways enriched by upregulated and downregulated DEGs, respectively.