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. 2022 Feb 8;12:820463. doi: 10.3389/fendo.2021.820463

Figure 8.

Figure 8

Validation of RNA-seq data using qRT-PCR. In total, expressions of 15 genes were detected by RNA-seq (red column) and qRT-PCR (blue column). (A) The expressions of gad2, oxt, tac1, scg2a, npy, ghrh-lr, gnrh3, kiss2, and zp3l genes in the brain and testes at different stages during gonadogenesis in common carp. (B) The expressions of sox30, fanci, dmrt1, dmc1, piwil, and hsd11b2 genes in the testis across gonad development.