Fig. 3.

LINC00930 correlates with and regulates PFKFB3. a The transcripts of glucose metabolism, TCA and oxidative phosphorylation (OXPHOS)-related genes were detected by real-time PCR in CNE1 cells with LINC00930 overexpression and CNE2 cells with LINC00930 knockdown. b The correlation between LINC00930 transcript level and PFKFB3 mRNA level was measured in cohort 1 (n = 71, spearman rank-correlation analysis). c and d The PFKFB3 expression was detected with real-time PCR and western blot in NPC cells with LINC00930 knockdown or LINC00930 overexpression. e Luciferase reporter vector was generated by inserting the promoter region (− 2000 bp to + 200 bp) of the PFKFB3 gene. The reporter vectors were then co-transfected into 5-8F and CNE2 cells with LINC00930 or control shRNAs. Cells were harvested for luciferase activity assay. f and g Intracellular F-2,6-BP level and PFK1 activity were assayed in NPC cells with LINC00930 knockdown. The p-value in c, d, e, f & g was were determined by a two-tailed unpaired Student’s t test. * p < 0.05; ** p < 0.01; *** p < 0.001