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. 2022 Feb 23;13:80. doi: 10.1186/s13287-022-02723-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization of TDSCs and TDSC-Exos. A Morphology of TDSCs and tenocytes. Scale bar: 50 μm. B Colony forming assay showed that TDSCs could form colony at a low cell density. C TDSCs exhibited multi-potential differentiation capacity for adipogenesis, osteogenesis and chondrogenesis. Scale bar: 50 μm. D Flow cytometric analysis of cell surface markers of TDSCs. Blue curves represent isotype controls, and red curves represent measured surface markers of TDSCs. E Morphology of TDSC-Exos observed by TEM. Scale bar: 100 nm. F The size distribution of TDSC-Exos peaks at 138.8 nm. G Exosomal markers (CD9, CD63, CD81 and TSG101) in TDSC-Exos were measured by western blotting