Table 2.
List of primers sets used for qPCR and RT-PCR analyses
| Primer id | Nucleotide sequence (5′ → 3′) | Target gene | Target group | Reaction | Reference |
|---|---|---|---|---|---|
| Eub338 (F) | ACCTACGGGAGGCAGCAG | 16S rRNA | Bacteria | qPCR | [39] |
| Eub518 (R) | ATTACCGCGGCTGCTGG | 16S rRNA | Bacteria | qPCR | [50] |
| FF390 (F) | CGATAACGAACGAGACCT | 18S rRNA | Fungi | qPCR | [73] |
| FR1 (R) | AICCATTCAATCGGTAIT | 18S rRNA | Fungi | qPCR | [73] |
| FsTri5 (F) | TGGAGAACTGGATGGTCTGG | Tri5 | TpFs | RT-qPCR | [2] |
| FsTri5 (R) | GACATAGCCGTGCATGAAGC | Tri5 | TpFs | RT-qPCR | [2] |
| FsTri4 (F) | GCCACTGCTGCTACTGTTGA | Tri4 | TpFs | RT-qPCR | [2] |
| FsTri4 (R) | GGTCGTTGTCCAGATGTTCTTG | Tri4 | TpFs | RT-qPCR | [2] |
| EF1A (F) | GGCTTTCACCGACTACCCTCCTCT | EF1A | Eukaryotes | RT-qPCR | [32] |
| EF1A (R) | ACTTCTCGACGGCCTTGATGACAC | EF1A | Eukaryotes | RT-qPCR | [32] |
F – Forward primer
R – Reverse primer
TpFs – Trichothecene producing Fusarium species (especially characterized in F. graminearum and F. sporotrichioides)