Fig. 2.

PAX8 promotes proliferation, migration and invasion of trophoblast cells. a Schematic diagram of miR-326 and its putative binding sequence in the wild-type (WT) and mutant (MUT) 3′-UTR of PAX8. b Western blot analysis to validate the efficiency of stably transfected cells. c, d, e CCK8 assays, transwell assays and colony formation assays from indicated cells with PAX8 knockdown or overexpression f Western blot analysis for YAP and TAZ protein in indicated cells. g Relative Hippo pathway activity. Renilla activity was used to normalize luciferase reporter activity