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. 2022 Jan 12;16(2):1791–1801. doi: 10.1021/acsnano.1c04681

Figure 2.

Figure 2

(A) Time-dependent fluorescence changes of Zn(II)-PPIX/G-quadruplex chains generated by miRNA-21-responsive Zn(II)-PPIX-loaded Ha/Hb-locked NMOFs treated with (a) PBS, (b) HEPES buffer. 0.1 mg NMOFs are treated with 100 μL of PBS or HEPES buffer, 10 mM, miRNA-21, 200 nM, and K+-ions, 50 mM. (B) Fluorescence spectra of Zn(II)-PPIX/G-quadruplex chains generated after a fixed time-interval of 15 min using variable concentrations of K+: (a) 0 mM K+, (b) 10 mM K+, (c) 20 mM K+, (d) 50 mM K+, (e) 100 mM K+, (f) 140 mM K+. 0.1 mg NMOFs are treated with 100 μL of PBS, 10 mM, miRNA-21, 200 nM. (C) Fluorescence spectra of Zn(II)-PPIX/G-quadruplex chains generated after a fixed time interval of 15 min using variable concentrations of miRNA-21: (a) 0 nM, (b) 100 nM, (c) 200 nM, (d) 500 nM, (e) 1 μM, (f) 2 μM. 0.1 mg NMOFs are treated with 100 μL of PBS, 10 mM, K+-ions, 50 mM. (D) Fluorescence spectra of Zn(II)-PPIX/G-quadruplex chains generated after a fixed time-interval of 15 min using variable concentrations of PBS: (a) 0 mM, (b) 5 mM, (c) 7.5 mM, (d) 10 mM, (e) 20 mM, (f) 50 mM. 0.1 mg NMOFs are treated with 100 μL of PBS, 10 mM, miRNA-21, 200 nM, and K+-ions, 50 mM. (E) Fluorescence spectra of Zn(II)-PPIX/G-quadruplex chains generated using different time intervals for operating the HCR process: (a) 0 min, (b) 15 min, (c) 30 min, (d) 60 min, (e) 120 min. 0.1 mg NMOFs are treated with 100 μL of PBS, 10 mM, miRNA-21, 200 nM, and K+-ions, 50 mM. (F) Fluorescence spectra of Zn(II)-PPIX/G-quadruplex chains generated using (a) miRNA-21, 200 nM; (b) miRNA-221, 200 nM; (c) miRNA-145, 200 nM. 0.1 mg NMOFs are treated with 100 μL of PBS, 10 mM, and K+-ions, 50 mM. Error bars derived from N = 3 experiments.