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. 2021 May 6;15(2):482–498. doi: 10.1111/1751-7915.13814

Fig. 2.

Fig. 2

Ponatinib transforms fluconazole from fungistatic into fungicidal and suppresses hyphal formation.

A. C. albicans (SC5314) cells were inoculated into YPD liquid medium supplemented with or without indicated compounds (DMSO, 32‐128 μg/ml fluconazole, 32‐128 μM ponatinib or both). The survival colony‐forming units (CFUs) of SC5314 were counted after incubation at indicated periods of time (0, 24 and 48 h).

B. C. albicans (SC5314) cells were revived and resuspended in each of three different hyphae‐inducing media (M199 buffered to pH8 with 50 mM MOPS; Spider; and YPD with 10% serum) containing DMSO, fluconazole (2 μg ml‐1), ponatinib (5 μM) or the combination. Cells were incubated at 37 °C, and hyphal morphology was checked under light microscope after 4 h of incubation.