Fig. 1.

Identification of IS5 in a plant binary vector by restriction digest.

A. Restriction digest of plasmid 8 isolated from two individual colonies derived from an original glycerol stock received from the donor of the plasmid with five restriction enzymes. EcoRI digestions are marked, showing an additional EcoRI site in colony 1. The centre lane shows the 1 Kb Plus Ladder (Invitrogen) as a marker.

B. Schematic representation of plasmid 8, showing the location of the restriction sites for the enzymes used to digest colonies 1 and 2 as shown in A. The location of the IS insertion with an additional EcoRI site is shown in colony 1.