Fig. 2.

IS elements are present in plant binary vectors with different backbones.

A. PCR screening of a pCambia‐based binary vector series using primers flanking the putative IS insertion site. (‐) Represents negative control (water); (+) represents positive control (plasmid 1).

B. PCR screening of pFGC5941 (plasmid 26) and pBIN20 (plasmid 27) derivatives using primers flanking the putative IS insertion site. In both sections A and B, a PCR product of ~1400 bp indicates the presence of an IS insertion (+IS) while a product of ~200 bp indicates the absence of IS insertion (no IS). A non‐specific PCR product is marked with an asterisk.