Fig. 3.

IS transposition occurs prior to stock deposition. PCR screening of a pCambia‐based binary vector series using primers flanking the putative IS insertion site. Plasmids isolated from 16 different stocks (P28–P43) were screened to detect the presence of an IS element. A PCR product of ~ 1400 bp indicates the presence of an IS insertion (+IS) while a product of ~ 200 bp indicates the absence of IS insertion (no IS). A non‐specific PCR product is marked with an asterisk.