Table 1.
Plasmid | AbR | Size | Rep. origin | Copy no. | Ratio of correct restriction pattern a |
---|---|---|---|---|---|
Plasmid 27 | Km | 14.4 kb | IncP | High | 9:10 |
Plasmid 44 | Km | 11.3 kb | ColE1 | High | 8:10 |
Plasmid 45 | Km | 8.6 kb | ColE1 | High | 0:10 |
Plasmid 46 | Km | 6.8 kb | ColE1 | High | 10:10 |
AbR, antibiotic resistance; Km, Kanamycin.
a
Restriction digestion of plasmid preparations made by culturing 10 colonies obtained after plating the respective stab culture.