Fig. 1.

Innate immune cell decidual infiltrates following COVID-19 in pregnancy. (A) Representative images demonstrating focused areas of decidual tissue for fluorescent microscopy analysis. D: Decidua, V: Villous tissue, C: chorionic plate. Black square denotes area of enlarged H&E image. Dotted outline denotes representative area of focused decidual tissue survey. Scale bar: 200 µm. (B, D) Representative images (200x) of decidual areas stained for (B) CD14 (green) or (D) CD56 (green) immunofluorescence. White scale bar = 50 µm. Dashed insets are higher magnification, with red scale bar = 12.5 µm. Solid insets: secondary-only controls. (C, E) Graphical analysis of comparative fluorescence quantitation of (C) CD14 or (E) CD56. Red symbols indicate placentas with positive staining for SARS-CoV-2 Spike protein. Star symbol indicates placenta from participant who required hospitalization for COVID-19. 2nd = 2nd Tri COVID (n = 8); 3rd = 3rd Tri COVID (n = 8); control/ctrl = negative for COVID (n = 8). ** p < 0.01; ** p < 0.001. Scale bar: 50 µm; insets: secondary-only controls.(For interpretation of the references to colour in this figure, the reader is referred to the web version of this article.)