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. 2022 Feb 10;10:813370. doi: 10.3389/fcell.2022.813370

FIGURE 2.

FIGURE 2

HAR inhibited myocardial apoptosis and alleviated mitochondrial injury in DICT mice. (A,B) Representative images and quantitative analysis of apoptosis by TUNEL in mouse cardiac tissues (n = 6). Scale bar: 20 μm. (C–E). Representative immunoblots and quantitative analysis of the expression of apoptosis-related proteins mediated by p53 shown as relative protein expression after normalization to GAPDH (n = 3–6). (F,G,H) Representative electron micrographs displaying mitochondrial arrangement and apoptosis bodies pointed by yellow arrow, and the quantification of mitochondria and apoptosis bodies in mouse hearts. Scale bar: 2.5 μm. The number of mitochondria and apoptosis bodies were quantified blindly using 10 images from 6 samples. (I,J,K) Representative images and quantitative analysis of ROS (DHE staining) and damaged mtDNA in mouse cardiac tissues (n = 6). Scale bar: 20 μm. Colocalization of 8-OhdG (DNA damage marker) and TOM20 (mitochondrial locator) was used to assess mtDNA damage. (L, M) The contents of serum MDA and SOD (n = 6). *p < 0.05, **p < 0.01, ***p < 0.001 vs. DOX group. The data are shown as the mean ± s.d.