Figure 3. Phylogenetic relationships, motifs and gene structures of laccase members from eggplant.

(A) The phylogenetic tree was constructed using the maximum-likelihood (ML) method with 1,000 replications, based on the multiple alignments of 48 laccase amino acid sequences from eggplant. The eight major groups were indicated and were differentiated with diverse colored backgrounds. (B) Gene structures of LAC genes from eggplant. CDS, UTR and introns were shown by green boxes, yellow boxes and black lines, respectively. (C) Schematic of the conserved motifs in the LAC proteins from eggplant elucidated by TBtools. Every amino acid motif in the SmLAC proteins (1–10) was represented by one colored box with a number. The black lines indicated relative protein lengths. Conserved motifs were defined by MEME. (D) Schematic of the conserved domains in the LAC proteins from eggplant elucidated by TBtools. The three conserved domains including Cu-oxidase, Cu-oxidase_2 and Cu-oxidase_3 were shown by yellow boxes, pink boxes and green lines, respectively. Conserved domains were defined by SMART.