Table 3.
Resistome of MDR hospital wastewater E. Coli 1000C-3 isolate.
| Antibiotic Resistance Genes | Location | Accession # | Resistance Target |
|---|---|---|---|
| aac(6’)-Ib-cr | Plasmid | DQ303918 | Aminoglycoside and fluoroquinolone resistance |
| aadA5 | Plasmid | AF137361 | Aminoglycoside resistance |
| aph(3’’)-Ib | Plasmid | AF321551 | Aminoglycoside resistance |
| aph(6)-Id | Plasmid | M28829 | Aminoglycoside resistance |
| blaCTX-M-15 | Plasmid | AY044436 | Beta-lactam resistance |
| blaOXA-1 | Plasmid | HQ170510 | Beta-lactam resistance |
| blaKPC-2 | Plasmid | AY034847 | Beta-lactam resistance |
| blaTEM-350 | Plasmid | WP045286946 | Beta-lactam resistance |
| mdf(A) | Chromosomal | Y08743 | Macrolide resistance |
| mph(A) | Plasmid | D16251 | Macrolide resistance |
| catB3 | Plasmid | AJ009818 | Phenicol resistance |
| sul1 | Plasmid | U12338 | Sulphonamide resistance |
| tet(A) | Plasmid | AJ517790 | Tetracycline resistance |
| tet(B) | Chromosomal | AF32677 | Tetracycline resistance |
| dfrA17 | Plasmid | FJ460238 | Trimethoprim resistance |
| sitABCD | Plasmid | AY598030 | Disinfectant resistance |
| qacE | Plasmid | X68232 | Disinfectant resistance |
| bacA | Chromosomal | 3002986 | Peptide antibiotic resistance |
| mdtG | Chromosomal | 3001329 | MFS antibiotic efflux pump |
| mdtH | Chromosomal | 3001216 | MFS antibiotic efflux pump |
| mdtP | Chromosomal | 3003550 | MFS antibiotic efflux pump |
| mdtN | Chromosomal | 3003548 | MFS antibiotic efflux pump |
| tolC | Chromosomal | 3000237 | MFS antibiotic efflux pump |
| emrA | Chromosomal | 3000027 | MFS antibiotic efflux pump |
| emrB | Chromosomal | 3000074 | MFS antibiotic efflux pump |
| emrK | Chromosomal | 3000206 | MFS antibiotic efflux pump |
| emrY | Chromosomal | 3000254 | MFS antibiotic efflux pump |
| evgA | Chromosomal | 3000833 | MFS antibiotic efflux pump |
| H-NS | Chromosomal | 3000676 | MFS antibiotic efflux pump |
| ampH | Chromosomal | 3004612 | ampC-type beta-lactamase |
| ampC1 | Chromosomal | 3004611 | ampC-type beta-lactamase |
| cpxA | Chromosomal | 3000830 | RND Efflux pump |
| gadX | Chromosomal | 3000508 | RND Efflux pump |
| mdtE | Chromosomal | 3000795 | RND Efflux pump |
| mdtF | Chromosomal | 3000796 | RND efflux pump |
| acrA | Chromosomal | 3004043 | RND Efflux pump |
| acrB | Chromosomal | 3000216 | RND Efflux pump |
| acrD | Chromosomal | 3000491 | RND Efflux pump |
| baeS | Chromosomal | 3000829 | RND Efflux pump |
| baeR | Chromosomal | 3000828 | RND Efflux pump |
| marA | Chromosomal | 3000263 | RND Efflux pump |
| yojI | Chromosomal | 3003952 | Peptide antibiotic efflux pump |
| pmrF | Chromosomal | 3003578 | Phosphoethanolamine transferase |
| kdpE | Chromosomal | 3003841 | Aminoglycoside efflux pump |
| msbA | Chromosomal | 3003950 | Nitroimidazole efflux pump |
| Point mutations | PMID | ||
| parC AGC-> ATC | 8851598 | Quinolone resistance | |
| parE TCG -> GCG | 28598203 | Quinolone resistance | |
| gyrA TCG -> TTG | 8891148 | Quinolone resistance | |
| gyrA GAC-> AAC | 12654733 | Quinolone resistance |
The following ARGs and mutations were identified by ResFinder 4.1 from the Center for Genomic Epidemiology and Comprehensive Antibiotic Resistance Database. MFS: major facilitator superfamily; RND: resistance nodulation-cell division.