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Nrf2 and Keap1 purified proteins aggregate upon exposure to oxidative stress in vitro. Purified protein treated with 600 μM H2O2 ± βME for 30 min for (A) full-length Nrf2, (B) Nrf2′s Neh5 domain, (C) full-length Keap1, and (D) Keap1′s Kelch domain. (E) Fractionation assay for purified full-length Nrf2 and Keap1 protein. All purified proteins were resolved using SDS-PAGE with Coomassie Blue gel staining and/or by SDD-AGE with western blot detection, as indicated.
