Figure 2.

Nrf2 is required for optimal macrophage-mediated efferocytosis. BMDMФs from Nrf2^+/+ (WT) and Nrf2^−/− mice were cultured and exposed to hyperoxia for 24 h. (a) Macrophages (green) were then incubated with labeled apoptotic neutrophils (red) for 1 h, washed, and captured images. (b) Macrophages were incubated with green fluorescent beads. Representative images of macrophages with neutrophils or beads for each genotype and experimental condition are shown. Values represent from at least three independent samples. * Room air versus hyperoxia of respective genotypes, †, Nrf2^−/− versus WT counterparts. * p < 0.05; **/†† p < 0.01; **** p < 0.0001. Blue, room air group; Red, hyperoxia group.