Figure 3.

Nrf2-deficiency in neutrophils does not affect their engulfment by macrophages. Neutrophils from the bone marrow of wild-type (WT) and Nrf2^−/− mice were isolated, labelled, and subjected to apoptosis as detailed in methods. Apoptotic neutrophils (red) were incubated with WT BMDMФs and Nrf2^−/− BMDMФs for 1 h, and efferocytosis was quantified. (a) Representative images of macrophages with apoptotic neutrophils (red). The blue color represents DAPI. (b) quantification of efferocytosis. Values are from 3–4 fields of two independent samples. † p < 0.05; Nrf2^−/− versus WT counterparts. Blue, room air group; pink hyperoxia group.