Figure 3.

SOD2-overexpressing cells showed an increase in amino-acid synthesis pathways and a partial Krebs Cycle blockage through a decrease in succinate dehydrogenase. Metabolomic analysis of LNCaP-Mock (MOCK) and LNCaP-SOD2 (SOD2). (A) Summary graph and list of the main metabolic pathways altered in SOD2 cells, showing impact and enrichment. (B) Relative uptake of ¹³C by succinate and malate, indicating accumulation of recently formed succinate (m + 4) and a decrease in fumarate. (C) Protein levels of succinate dehydrogenase (SDH) in MOCK and SOD2 cells, values of densitometric quantification relative to B-ACTIN are shown. (D) Levels of lactate loaded with 1-, 2-, or 3-¹³C in its structure (n = 3 each group). For all panels, values are expressed as mean ± SEM; t-test was used as statistical analysis; * = p-value < 0.05, ** = p-value < 0.01.