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OMF–PAP interactions assessed by BLI. Immobilized BNAPol-OprM (A,B), BNAPol-OprN (C,D), BNAPol-TolC (E,F), BNAPol-OprM∆473−485 (G,H) were exposed to different concentrations (from 0 to 100 µM) of MexAwt (left column) or MexAQ93R (right column). Interactions (association and dissociation) were assessed by a wavelength shift (nm). All reactions were performed at room temperature.
