Table 1.

Kinetics parameters for the OMF–PAP interaction using biolayer interferometry.

Ligand	Analyte	koff (10−3 s−1)	kon (102 M−1s−1)	KD (µM)
OprMwt	MexAwt	2.15	1.80	12.0
OprM∆473−485	MexAwt	4.58	1.03	44.0
OprN	MexAwt	3.58	1.77	20.0
TolC	MexAwt	5.8	1.27	45.8
OprMwt	MexAQ93R	2.66	0.81	32.9
OprM∆473−485	MexAQ93R	2.63	1.02	25.7
OprN	MexAQ93R	2.38	0.88	26.9
TolC	MexAQ93R	1.91	1.08	17.8

Data fitting using Langmuir 1:1 model.