Table 3.
Predicted means and associated standard error of the mean of organic acids and ammonia concentrations following in vitro fermentation of each substrate at the 24-h time point in the CD (high carbohydrate) faecal inoculum. Letter-based representation of pairwise comparisons is presented. Different letters indicate differences at significance level 0.05, adjusted using the Tukey method. AHC; ANZCO hydrolysed collagen. PHC; Peptan hydrolysed collagen.
| Acetate (µmol/mL) | Butyrate (µmol/mL) | Propionate (µmol/mL) | Total SCFA (µmol/mL) | |||||||||
| Substrate | Mean | SEM | Comparison | Mean | SEM | Comparison | Mean | SEM | Comparison | Mean | SEM | Comparison |
| Control | 16.867 | 0.218276 | DE | ND | ND | C | 0.55 | 0.140 | B | 30.46 | 1.243959 | BCD |
| AHC | 17.99 | 0.555908 | CDE | ND | ND | C | 0.63 | 0.166 | B | 32.69 | 2.010075 | ABC |
| PHC | 15.85 | 0.20664 | EF | ND | ND | C | 0.59 | 0.152 | B | 28.1233 | 0.902244 | CD |
| Cellulose | 12.783 | 0.239188 | F | ND | ND | C | 0.42 | 0.012 | B | 24.24 | 0.574717 | D |
| Freeze-dried Cartilage | 22.873 | 1.264283 | A | ND | ND | C | 0.51 | 0.058 | B | 37.5167 | 1.573503 | A |
| Fresh Cartilage | 20.767 | 1.120897 | ABC | ND | ND | C | 0.48 | 0.041 | B | 36.7867 | 2.106255 | AB |
| Chopped cat hair | 19.54 | 0.62426 | BCD | 3.027 | 0.292252 | B | 2.56 | 0.214 | A | 30.4733 | 1.140663 | BCD |
| Intact cat hair | 22.127 | 0.139084 | AB | 4.33 | 0.555108 | A | 3.04 | 0.100 | A | 34.5367 | 0.398427 | ABC |
| Inulin | 16.417 | 0.096148 | DE | 1.053 | 0.027285 | C | 0.57 | 0.123 | B | 31.8 | 0.831164 | ABC |
| Formate (µmol/mL) | Lactate (µmol/mL) | Succinate (µmol/mL) | Ammonia (mM) | |||||||||
| Mean | SEM | Comparison | Mean | SEM | Comparison | Mean | SEM | Comparison | Mean | SEM | Comparison | |
| Control | 11.933 | 1.281748 | AB | 0.783 | 0.269093 | C | 0.517 | 0.048 | D | 17.533 | 0.0805 | A |
| AHC | 12.963 | 1.413416 | AB | 0.563 | 0.165965 | C | 0.997 | 0.033 | C | 13.859 | 0.2324 | BC |
| PHC | 10.573 | 1.068431 | AB | 0.743 | 0.279543 | C | 1.320 | 0.032 | C | 12.958 | 0.0869 | C |
| Cellulose | 9.937 | 0.346041 | B | 1.04 | 0.280951 | C | 0.59 | 0.038 | D | 14.533 | 0.2932 | B |
| Freeze-dried Cartilage | 12.977 | 0.302067 | AB | 5.487 | 0.086859 | B | 2.2 | 0.040 | A | 12.463 | 0.41347 | C |
| Fresh Cartilage | 14.393 | 1.083349 | A | 5.183 | 1.036345 | B | 2.287 | 0.168 | A | 12.983 | 0.03876 | C |
| Chopped cat hair | 5.243 | 0.188532 | C | 2.373 | 0.315454 | C | 0.37 | 0.070 | D | 17.02 | 0.4105 | A |
| Intact cat hair | 4.937 | 0.127061 | C | 2.167 | 0.384765 | C | ND | ND | D | 17.578 | 0.43091 | A |
| Inulin | 13.65 | 0.700595 | AB | 22.593 | 0.929737 | A | 1.717 | 0.046 | B | 10.799 | 0.2829 | D |