Figure 4.

RCAN1-mediated immunosuppression in AD mice. Wild-type (WT) and RCAN1 knockout mice (RCAN1-KO) (n = 5–7 per group) were treated with oxazolone together with either vehicle (DMSO) or CA extract (10 μg/mL). (A) Ear thickness was measured with a micrometer. Data represent mean ± SD (n = 5–7). (B) Formalin-fixed ear tissue sections were stained with hematoxylin and eosin (H&E). Bars: 50 μm. (C) The mRNA levels of IL-6, TNF-α, and β-actin were measured from ear tissues using RT-PCR and quantified. The graphs are the means ± SD of three independent experiments. n.s. (p > 0.05), * p < 0.05, ** p < 0.01 compared with indicated group.