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. 2022 Feb 4;11(2):314. doi: 10.3390/antiox11020314

Figure 6.

Figure 6

Epac1 inhibition prevents intracellular soluble ROS production. (A) Overlay of gated cells of soluble ROS production under 1 µM erastin-induced ferroptosis. (B) Representative graph of the soluble ROS production under 1 µM erastin-induced ferroptosis and Epac1 inhibition using 100 μM CE3F4. (C) Overlay of gated cells of soluble ROS production under 1.5 µM erastin-induced ferroptosis. (D) Representative graph of the soluble ROS production under 1.5 µM erastin-induced ferroptosis and Epac1 inhibition using 100 μM CE3F4. Control is DMSO 1%. * Comparison of the group against control, # Comparison between different treatment groups. n ≥ 3 biological replicates with n = 3 technical replicates for each condition and biological replicate. One-Way ANOVA statistical analysis with Bonferroni correction was used. ns p > 0.05, *** p < 0.001, **** p < 0.0001. ### p < 0.001, #### p < 0.0001.