Table 1.
Primer Sequence (5′–3′) | ||||
---|---|---|---|---|
Gene | Accession Number | Forward | Reverse | Amplicon Size (bp) |
CAT | NM_012520.2 | ACAACTCCCAGAAGCCTAAGAATG | GCTTTTCCCTTGGCAGCTATG | 76 |
Caspase-8 | NM_022277.1 | GTTCTCTCAGTTGCCTTTCTCC | GGCCAGTCCGCCAAAGTTTA | 90 |
IL-10 | NM_012854.2 | TTGAACCACCCGGCATCTAC | CCAAGGAGTTGCTCCCGTTA | 91 |
Inos | XM_006246949.3 | CAGCCCTCAGAGTACAACGAT | CAGCAGGCACACGCAATGAT | 91 |
SOD | X05634.1 | CGAGCATGGGTTCCATGTC | CTGGACCGCCATGTTTCTTAG | 101 |
IL-1b | NM_031512.2 | GACTTCACCATGGAACCCGT | GGAGACTGCCCATTCTCGAC | 104 |
p53 | NG_005120.4 | CTACTAAGGTCGTGAGACGCTGCC | TCAGCATACAGGTTTCCTTCCACC | 106 |
Nrf2 | NM_031789.1 | CAGGTTGCCCACATTCCCAA | ATATCCAGGGCAAGCGACTCAT | 110 |
Bax | U49729.1 | CGCGTGGTTGCCCTCTTCTACTTT | CAAGCAGCCGCTCACGGAGGA | 129 |
Bcl-2 | NM_016993.1 | ATCGCTCTGTGGATGACTGAGTAC | AGAGACAGCCAGGAGAAATCAAAC | 134 |
GPx | NM_030826.4 | GGAGAATGGCAAGAATGAAGA | CCGCAGGAAGGTAAAGAG | 139 |
TNF-a | NM_012675.3 | ACTGAACTTCGGGGTGATCG | GCTTGGTGGTTTGCTACGAC | 153 |
NF-kB(p65) | NM_199267.2 | CGCGGGGACTATGACTTGAA | AGTTCCGGTTTACTCGGCAG | 163 |
Caspase-9 | NM_031632 | CTGAGCCAGATGCTGTCCCATA | CCAAGGTCTCGATGTACCAGGAA | 168 |
GAPDH | NM_017008 | TCACCACCATGGAGAAGGC | GCTAAGCAGTTGGTGGTGCA | 169 |
Caspase-3 | NM_012922 | AAGATACCAGTGGAGGCCGACTTC | GGGAGAAGGACTCAAATTCCGTGG | 199 |
CAT: catalase; iNOS; inducible nitric oxide synthase; SOD: superoxide dismutase; IL: interleukin; p53: tumour suppressor; Nrf2: nuclear factor erythroid 2-related factor 2; Bcl2: B-cell lymphoma 2; Bax: Bcl-2-associated X protein; GPx: glutathione peroxidase; TNF: tumour necrosis factor; NF-kB: nuclear factor kappa B; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.