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. 2022 Jan 21;11(2):203. doi: 10.3390/antiox11020203

Table 1.

Different green extraction used to obtain bioactives from tropical fruit by-products.

Fruit By-Product Composition Solvent Extraction Method Characterization Method Major Compounds Extracted/Identified Ref.
Mango Seed Kernel EtOH (50%) Agitation for 1 h at room temperature and 2000 rpm HPLC-UV/Vis Gallic acid, caffeic acid, rutin, Penta-O-galloyl-b-D-glucose, galloylglucose species with galloyl moieties (greater than five) [67]
Peel EtOH UAE at 25 kHz; MAE at 2450 mHz HPLC/ESI/MS Phenolic compounds and flavonoids [68]
Lactic acid,
NaOAc and
H2O (3:1:4)
MAE at 436.45 W, 19.66 min HPLC Mangiferin [69]
EtOH MAE + UAE (2450 MHz and 25 KHz, respectively) HPLC/ESI/MS Polyphenols [70]
Pineapple Core Phosphate buffer Agitation MALDI-TOF-TOF Glycylendopeptidases [71]
H2O Autohydrolysis HPLC Gallic, hydroxybenzoic, chlorogenic, coumaric and caffeic acids and epicatechin [72]
Rind N-Hexane Cold extraction GC-MS Limonene, alpha-farnesene, trans caryophyllene and myrcene [73]
Avocado Seed EtOH MAE at 71.64 °C for 14.69 min HPLC-ESI-MS Perseitol, procyanidins, hydroxytyrosol glycoside, caffeoylquinic acid, coumaroylquinic acid, catechin and epicatechin [74]
EtOH/H2O (80:20, v/v) UAE for 15 min at 25 °C HPLC-MS/MS Procyanidin B1, catechin, epicatechin and trans-5- O-cafeoyl-D-quinic acid [26]
Peel EtOH (36%) MAE for 39 min at 130 °C HPLC-ESI-TOF/
QTOF-MS
Quinic acid, citric acid, syringic acid, procyanidins, catechin, epicatechin, rutin, quercetin derivates, naringenin and kaempferol [75]
MeOH (80%) Maceration at 15 °C for 24 h UPLC-ESI-MS/MS Procyanidins, quinic acid, citric acid, catechin, epicatechin, rutin, quercetin, caffeic acid, ferulic acid and kaempferol [76]
EtOH/H2O (50:50, v/v) PLE at 200 °C for 20 min at 11 MPa HPLC-DAD-ESI-TOF-MS Pyrocatechol, vanillic acid, catechin, epicatechin, procyanidins, rutin, quercetin, kaempferol, sakuranetina and naringenin [77]
Custard Apple Seed EtOH (80%) Agitation at 200 rpm for 1 week GC-MS β -stigmasterol, β -sitosterol and dihydrobrassicasterol [41]
MeOH/H2O (80:20, v/v) UAE for 15 min at room temperature HPLC-DAD-ESI-QTOF-MS Catechin, epicatechin, procyanidins, vanillic acid, quinic acid, hydroxybenzoic acid and syringic acid [78]
Peel MeOH (15%) SFE with CO2 for 3 h at 75 °C and 100 bars UPLC-ESI-MS Aporphine, boldine, flaucine, anonaine, xilopine and pehnolamide [79]
EtOH (70%) Agitation in the dark for 12 h at 4 °C and 120 rpm LC-ESI-QTOF-MS/MS and HPLC-PDA Kaphtaric acid, chlorogenic acid, p-hydroxybenzoic acid, coumaric acid, ferulic acid, sinapinic acid and resveratrol [80]
Leaves EtOH/H2O (75:25, v/v) Maceration for 3 days in the dark at room temperature HPLC-ESI-DAD-MS/MS Catequin, epicatechin, kaempferol-3- O- glucoside, anonaine, quercetin-3- O -glucoside and pronuciferin [44]
EtOH/H2O (80:20) and Acetone/H2O (70:30) UAE for 20 min HPLC-ESI-TOF-MS Neochlorogenic acid, rutin, epicatechin, catechin, procyanidins, trigonelline, quinic acid, citric acid, kaempferol and quercetin [81]
Papaya Seed CO2-EtOH(5, 8%) SFE with CO2-EtOH for 180 min at 50 °C and 20 MPa HPLC-ESI-MS/MS Chlorogenic acid, caffeic acid, ferulic acid, p-hydroxybenzoic acid and p-coumaric acid [82]
Distilled Water SWE for 5 min at 150 °C LC-ESI-MS/MS Ferulic acid, mandelic acid, vanillic acid, caffeic acid, chlorogenic acid and myricetin [83]
Peel EtOH (70%) Agitation for 12 h at 4 °C and 120 rpm LC-ESI-QTOF-MS/MS y HPLC-PDA Gallic acid, kaphtharic acid, p-hydroxybenzoic acid, syringic acid, ferulic acid, epicatechin, kaempferol and quercetin [80]
EtOH (23.32%) MAE for 3 min at 340 W GC-MS Phenolic compounds and flavonoids [84]
Leaves MeOH Maceration for 72 h at room temperature - Phytol, β-sitosterol, γ-tocopherol, δ-tocopherol and hexadecanoic acid [85]
H2O UAE for 10–20 min HPLC-DAD-QTOF-MS/MS Caffeoylquinic acid, coumaric acid derivatives, ferulic acid derivatives, rutin, kaempferol glucoside and rhamnoside [86]
Guava Seed MeOH Guava seed oil: Agitation for 1 min at 20 °C RP-UHPLC-DAD-HESI-MS/MS; GC-MS Vanillic acid, vanillin, syringaldehyde, abscisic acid and cinnamic acid, β-sitosterol, α-tocopherol, γ-tocopherol and campesterol [87]
EtOH/H2O
(30:70, v/v)
UAE for 2 min
at 25 °C, 20 kHz and 500 W
LC-ESI-MS/MS Salicylic acid, cinnamic acid, p-coumaric acid, vanillic acid, ferulic acid, ellagic acid, gallic acid, galangin, naringenin, catechin and quercetin [88]
Peel MeOH/H2O (9:1, v/v) +1% formic acid UAE UHPLC-DAD-MS/MS Gallic acid and derivatives, derivatives of cinnamic acid, caffeoylquinic acids, flavanols, proanthocyanidins, ellagitannins and anthocyanidins [89]
Leaves Distilled H2O Boiling at 90 °C for 30 min LC/MS Quercetin, 3-synapoylquinic acid, esculin, gallocatequin, ellagic acid, gallic acid and citric acid [90]
EtOH (70%) UAE for 30 min at 340 W HPLC-ESI-TOF-MS Rutin, quercetin, quercitrin, kaempferol and avicularin [91]
Litchi Seed EtOH (95%) Direct reflux extraction for 2 h at 90 °C HPLC Catechin, epicatechin, litchiol A and litchiol B [92]
Peel Phosphate buffer
Hot H2O
Enzyme extractionPressurized hot-water extraction HPLC Polyphenolic content [93]
Pass. Fruit Seed EtOH (70%) Agitation for 30 min at 80 °C HPLC-DAD
LC-ESI-MS/MS
Flavonoids [94]
Peel EtOH (70%) HAE for 2 min UHPLC-PDA Isoorientin, orientin and sovitexin [15]
Leaves Aqueous solutions of ionic liquids (ILs) IL-MA-SLE HPLC-PDA Rutin, quercetin and apigenin [95]

Abbreviations: EtOH (Ethanol), HPLC (High Performance Liquid Chromatography), Vis (Visible), UAE (Ultrasound-Assisted Extraction), MAE (Microwave-Assisted Extraction), ESI (Electrospray Ionization), MS (Mass Spectrum), NaOAc (Sodium Acetate), MALDI (Matrix-Assisted Laser Desorption/Ionization), TOF (Time Of Flight), GC (Gas Chromatography), QTOF (Quadrupole Time Of Flight), MeOH (Methanol), UPLC (Ultra Performance Liquid Chromatography), PLE (Pressurized Liquid Extraction), DAD (Diode-Array Detection), SFE (Supercritical Fluid Extraction), LC (Liquid Chromatography), PDA (Photodiode-Array Detection), SWE (Subcritical Water Extraction), RP (Reversed Phase), UHPLC (Ultra High Performance Liquid Chromatography), HESI (Heated Electrospray Ionization), HAE (Homogenizer-Assisted), IL-MA-SLE (Ionic Liquid Microwave-Assisted Solid-Liquid Extraction).