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Transfection efficiency of Drp1-siRNA in retinal endothelial cells. The transfection efficiency Drp1-siRNA transfected HRECs was determined by quantifying Drp1 (a) gene transcripts (qRT-PCR, β-actin as a housekeeping gene) and (b) protein expression (Western blot, β-actin as a loading protein). The values are represented as mean ± SD from 3 or more samples, each per-formed in duplicate. N and N/D-si are untransfected or Drp1-siRNA transfected cells, respective-ly; * p < 0.05 vs. N.
