Skip to main content
. 2022 Feb 11;11(2):365. doi: 10.3390/antiox11020365

Figure 4.

Figure 4

Mitochondrial translocation of Drp1. Mitochondrial localization was determined by immunofluorescence technique using Alexa Fluor–488 (green) conjugated secondary antibody for Drp1 and Texas red conjugated for the mitochondrial marker Tom20. DAPI-containing (blue) Vectashield mounting medium was used for mounting the cells, and the cells were imaged using a 63× oil objective lens on a ZEISS microscope. Pearson’s correlation coefficient between Drp1 and Tom20 was calculated using the colocalization ZEISS software module. The values in the graphs are presented as mean ± SD obtained from 3–4 different cell preparations. NG, cells in normal glucose; HG and HG + Hcy, cells in high glucose and high glucose + homocysteine respectively; HG/NIL and HG + Hcy/NIL, cells in high glucose containing NIL in the presence or absence of homocysteine, respectively; L-Gl, 20 mM L-glucose; * p < 0.05 compared to NG; ** p < 0.05 compared to HG; # p > 0.05 compared to NG.