Figure 7.

Effect of homocysteine on Drp1 nitrosylation. Drp1 nitrosylation was determined by following the conversion of 2,3-diaminonaphthalene to fluorescent 2,3-naphthyltriazole. The values presented are mean ± SD from 3–4 different cell preparations with each measurement made in duplicate: NG and HG, cells in normal and high glucose respectively; HG + Hcy, cells in high glucose + homocysteine; HG/NIL and HG + Hcy/NIL, cells in the presence of NIL in high glucose or high glucose + homocysteine, respectively; L-Gl, 20 mM L-glucose; * and ** p < 0.05 vs. NG and HG, respectively; # p > 0.05 vs. NG.