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. 2022 Feb 10;11(2):344. doi: 10.3390/antiox11020344

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Total cell number (TCN), trophectoderm cells (TE), inner cell mass (ICM), and ICM/TCN of differentially stained day 8 blastocyst before (A) and after vitrification-warming (B). Maturation media was supplemented with lycopene (0.2 μM final concentration), menadione (5 µM final concentration), lycopene + menadione, and no supplementation (control), and standard in vitro fertilization and culture were performed. Different superscripts (a, b, and c) represent statistical differences (p < 0.05) between experimental groups. Results are expressed as least square means ± standard errors.