Figure 3.

Total apoptotic cells (AC) and AC/total cell number (TCN) of differentially stained day 8 blastocyst before (fresh) and after vitrification and warming (post-warming). Maturation media was supplemented with lycopene (0.2 μM final concentration), menadione (5 µM final concentration), lycopene + menadione, and no supplementation (control), and standard in vitro fertilization and culture were performed. Different superscripts (a, b, and c) represent statistical differences (p < 0.05) between experimental groups. Results are expressed as least square means ± standard errors.