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. 2022 Feb 10;11(2):344. doi: 10.3390/antiox11020344

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Relative fluorescence units of oocytes in the maturation media supplemented with lycopene (L; 0.2 μM final concentration), menadione (M; 5 μM final concentration), lycopene + menadione (L+M), and no supplementation (control; C), and stained with CellROX^® to quantify the production of reactive oxygen species. Different superscripts (a, b, and c) represent statistical differences (p < 0.05) between experimental groups. (B) Representative images of the fluorescence intensities of oocytes matured in the maturation media with L, M, L+M, and no supplementation (C) and stained with CellROX^®.