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. 2022 Feb 10;11(2):344. doi: 10.3390/antiox11020344

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Plots showing the gene/set pathways, number of genes within the set/pathway, and the p-values of the differentially expressed gene/set pathways between lycopene-treated (0.2 μM final concentration) and non-treated (control) day 8 blastocysts. Lycopene treatment was performed during in vitro oocyte maturation. RNA-sequencing was performed from eight pools (four lycopene and four control) of ten blastocysts per pool. (A) Eighteen gene ontology (GO) terms were found to be differentially expressed using overrepresentation analysis (ORA). (B) Ten GO terms were found to be differentially expressed using pathway analysis with down-weighting of overlapping genes (PADOG). Pathways with p ≤ 0.05 were considered statistically significant. The gene sets of the differentially expressed GO terms were classified as biological process (BP), cellular component (CC), or molecular function (MF).