Figure 11.

The effects of Ecklonia cava extract on adipogenic and lipogenic gene expression. Total mRNA was extracted from different groups of rats, and qualitative gene expression was assessed by polymerase chain reaction (or endpoint PCR). Results obtained from different groups show that the high-fat diet-induced the up-regulation of (A) PPAR-γ (peroxisome proliferator-activated receptors gamma), (B) LPL (lipoprotein lipase), (C) FAS (fatty acid synthase), and (D) SREBP-1C genes that can be reduced by the treatment with E. cava ethanol (70%) extract. In figure, groups are symbolized as: NC (normal control), PC (positive control, i.e., HCA 250 mg/kg b.w), T1, T2, and T3 shows different doses of EC, i.e., 125, 250, and 500 mg/kg b.w, respectively, and HFD (high-fat diet).