Table 1.

The reaction system for PCR amplification.

Items	Volume
10 × TaKaRa rTaq buffer	2 μL
2.5 mM dNTPs	2 μL
5 μM forward primer	0.8 μL
5 μM reverse primer	0.8 μL
TrTaq Polymerase	0.2 μL
template DNA	10 ng
ddH2O	up to 20 μL