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. 2022 Feb 6;11(2):323. doi: 10.3390/antiox11020323

Figure 3.

Figure 3

Effect of lipopolysaccharide (LPS, 1.75 mg/kg), methyltriphenylphosphonium chloride (TPP, 50 nmol/kg) and mitoTEMPO (mT, 50 nmol/kg) on gene expression of iNOS (a), HO1 (b), IL-6 (c), TNFR1 (d), NFkBia (e), and CHOP (f) in rat liver. After LPS injection, gene expression levels of all mediators increased (a–f). MitoTEMPO treatment decreased the mean values of iNOS (a) and IL-6 (c). An increase is seen in IL-6 expression in the LPS+TPP group compared to the other LPS groups. All data are normalized for the expression levels of the reference genes (cyclophilin A and hypoxanthine-guanine phosphoribosyl transferase (HPRT)) and expressed as fold changes relative to the internal standard. * p < 0.05, ** p < 0.01, *** p < 0.001. One-way analysis of variance (ANOVA), Fisher’s least significant difference (LSD), n = 3–8, mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Abbreviations: inducible nitric oxide synthase (iNOS), heme oxygenase 1 (HO-1), interleukin 6 (IL-6), tumor necrosis factor receptor 1 (TNF-R1), nuclear factor kappa B inhibitor alpha (NFkBia), CCAAT/enhancer-binding protein homologous protein (CHOP).