Figure 4.

Effects of Na₂SeO₃ and L-SeMC on oxidative stress in PA-induced AML-12 cells. (A) ROS content detection in AML-12 cells after co-treatment with PA with or without Na₂SeO₃ and L-SeMC for 24 h, including images (400× magnification) and fluorescence density analysis (n = 3, * p < 0.05). (B) The apoptosis of AML-12 cells was determined by Annexin V/PI apoptosis staining kit after co-treatment with PA with or without Na₂SeO₃ and L-SeMC for 24 h (200× magnification). (C) Mitochondrial content was measured by Mito traker green after co-treatment with PA with or without Na₂SeO₃ and L-SeMC for 24 h (400× magnification). (D) Mitochondrial membrane potential was measured by JC-1 staining kit after co-treatment with PA with or without Na₂SeO₃ and L-SeMC for 24 h (400× magnification).