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Effect of 3-hydroxybutyrate (3-OHB) on NRF2 expression in HK-2 cells. (A) HK-2 cells were treated with/without 10 mM of 3-OHB and incubated for the indicated times. A representative figure showing expression levels of NRF2. (B) HK-2 cells were treated with/without 10 mM of 3-OHB and incubated for 12 h. Cellular RNA was extracted and NRF2 expression level was determined by real time-RT PCR. (C) Fluorescence images of NRF2 (green). DAPI, 4,6-diamidino-2-phenylindole; ** p < 0.01.
