Figure 6.

Differences in cardiolipin composition for brain mitochondria from iPLA₂γ-KO mice vs. WT. (A) Isolated mitochondria without further treatment, frozen instantly after isolation, (B) mitochondria isolated in the presence of NAC and respiring for 1 h in the presence of TBHP, and (C) mitochondria isolated in the absence of NAC and respiring for 1 h in the absence of TBHP. (D) The changes in cardiolipin composition of individual cardiolipin species of isolated WT and iPLA₂γ-KO mitochondria, without further treatment, frozen instantly after isolation (D). Isomers are marked with the number in parentheses. * p < 0.05; ** p < 0.01; # p < 0.001; $ p < 0.000001. Lipidomic data are collected from n = 6 replicates.