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. Author manuscript; available in PMC: 2022 Aug 14.
Published in final edited form as: Nat Cell Biol. 2022 Feb 14;24(2):194–204. doi: 10.1038/s41556-022-00844-9

Extended Data Fig. 3. β-integrin and talin expression analysis.

Extended Data Fig. 3

a, Expression analysis of β-integrins in the migrating primordium by in situ hybridization on 32 hpf embryos. Note that itgb8 could not be amplified from embryonic cDNA. Arrows indicate expression in the primordium. Scale bar = 0.5 mm. b, Schematics of the itgb1b locus, the itgb1b targeting cassette, and the modified itgb1b locus. c, Itgb1b-sfGFP expression in a 28 hpf itgb1b:itgb1b-sfGFP embryo. The image is a sum-projected z-stack. Scale bar = 0.5 mm. d, in situ hybridization against the three zebrafish talin genes on 32 hpf embryos. Arrow indicates enriched talin expression in the primordium. Scale bar = 0.5 mm. e, Schematic of the TgBAC(tln1:tln1-YPet) transgene and its protein product. f, Tln1-YPet expression in a 28 hpf tln1:tln1-YPet embryo. The image is a sum-projected z-stack. Scale bar = 0.5 mm.