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. Author manuscript; available in PMC: 2022 Aug 14.
Published in final edited form as: Nat Cell Biol. 2022 Feb 14;24(2):194–204. doi: 10.1038/s41556-022-00844-9

Fig. 7. The basement membrane wrinkles around the primordium.

Fig. 7.

a, F-tractin-mCherry distribution in the primordium (top) and LamC1-sfGFP around the primordium (bottom) in a 32 hpf embryo. LamC1-GFP fluorescence intensity is pseudo-colored as a heat map. The image is a maximum-projected z-stack. Scale bar = 50 μm. b, Transverse section through the F-tractin-mCherry-expressing primordium and the underlying LamC1-sfGFP-labeled BM (left top). Corresponding image showing the LamC1-sfGFP fluorescence intensity as a heat map (left bottom). Arrows indicate apposed clusters of F-tractin-mCherry and LamC1-sfGFP. Images are single sections along the YZ-plane of a z-stack. Scale bar = 10 μm. Fluorescent intensity profiles of F-tractin-mCherry and LamC1-sfGFP of image shown in left along the Y-axis (right). Arrows indicate the position of the apposed clusters of F-tractin-mCherry and LamC1-sfGFP indicated by arrows in left. c, Quantification of the LamC1-sfGFP intensity within 3 μm-wide bands around the perimeter of the primordium (left) and at a distance of 6 μm from the primordium’s perimeter (right). ****: p<0.0001 (two-tailed paired t-test). n = number of embryos.