Agarose gel electrophoresis of vanA, vanB, vanC-1, and vanC-2 PCR products. (A) Multiplex vanA and vanB gene PCR (lanes 2 to 7) and vanC-1 gene PCR (lanes 9 to 14). Lanes: 1 and 8, Amresco PCR DNA marker; 2, E. faecalis ATCC 51299 vanB, 297 bp (product size, 297 bp); 3, E. faecium wild strain vanA, 783 bp (product size, 783 bp); 4, E. gallinarum NCTC 11428 vanC-1, 822 bp (no product detected); 5, E. casseliflavus ATCC 25788 vanC-2, 439 bp (no product detected); 6, WBG 9213 (product size, 783 bp); 7, water control; 9, E. faecalis ATCC 51299 vanB, 297 bp (no product detected); 10, E. faecium wild strain vanA, 783 bp (no product detected); 11, E. gallinarum NCTC 11428 vanC-1, 822 bp (product size, 822 bp); 12, E. casseliflavus ATCC 25788 vanC-2, 439 bp (no product detected); 13, WBG 9213 (product size, 822 bp); 14, water control. (B) vanC-2 gene PCR. Lanes: 1, Amresco PCR DNA marker; 2, E. faecalis ATCC 51299 vanB, 297 bp (no product detected); 3, E. faecium wild strain vanA, 783 bp (no product detected); 4, E. gallinarum NCTC 11428 vanC-1, 822 bp (no product detected); 5, E. casseliflavus ATCC 25788 vanC-2, 439 bp (product size, 439 bp); 6, WBG 9213 (no product detected); 7, water control.