Table A2.
Primordial germ cell transplantation in fish.
| Donor | Enrichment | Recipient | % of Recipients with Gonadal Incorporation of Donor Cells |
% of Recipients Producing Donor-Derived Gametes | Survival of Recipients (%) | % of Donor-Derived Offspring |
Ref | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Species | Fish | Age | Cells | Method | Purity | Species | Fish | Age | Total | Male | Female | ||||
| A. japonica | WT | Somito-genesis | 1 PGC | MC | N/A | D. rerio | WT | Blastula | 42.7 at 2 d | N/A | N/A | N/A | Con: 92.7 TP: 92.6 at 2 d |
N/A | [161] |
| C. auratus | WT | 10–15 somite St | 1 PGC | MC | N/A | D. rerio | Dnd-KD | Blastula | N/A | N/A | Y at 10–12 mo | N | N/A | N/A | [16] |
| WT | 10–15 somite St | 1 PGC | MC | N/A | D. rerio | Dnd-KD | Blastula | 63.5 at 2 d | N/A | N/A | N/A | 69.0 at 2 d | N/A | [13] | |
| Albino | 10–15 somite St | 1 PGC | MC | N/A | C. auratus | Dnd-KD | Blastula | 42.7 at 3 d | 42.9 at 5 mo–2 y | 62.5 at 5 mo–2 y | 30.1 at 5 mo–2 y | Dnd-KD: 88.3 TP: 84.9 |
92.5–100 | [65] | |
| D. albolineatus | WT | 10–15 somite St | 1 PGC | MC | N/A | D. rerio | Dnd-KD | Blastula | 45.1 at 2 d | 89.5 at SM | 93.8 at SM | 66.7 at SM | Dnd-KD: 59.2 TP: 74.7 at 2 d |
100 | [16] |
| WT | 10–15 somite St | 1 PGC | MC | N/A | D. rerio | Dnd-KD | Blastula | 40.2 at 2 d | N/A | N/A | N/A | 76.8 at 2 d | N/A | [13] | |
| D. rerio | WT | 10–15 somite St | 1 PGC | FACS | 87.8–100 | D. rerio | Dnd-KD | Blastula | 31.5 at 1 dpt | N/A | N/A | N/A | Dnd-KD: 92.9 Dnd-KD + TP: 81.4 at 1 dpt |
N/A | [17] |
| WT | 10–15 somite St | 1 PGC | MC | N/A | D. rerio | Dnd-KD | Blastula | 30.0 at 2 d | N/A | N/A | N/A | 89.7 at 2 d | N/A | [13] | |
| 21–25 somite St | 10.1 at 2 d | N/A | N/A | N/A | 82.4 at 2 d | N/A | |||||||||
| Prim-5 St | 5.3 at 2 d | N/A | N/A | N/A | 78.4 at 2 d | N/A | |||||||||
| Prim-15 St | 4.5 at 2 d | N/A | N/A | N/A | 87.3 at 2 d | N/A | |||||||||
| WT | 10–15 somite St | 1 PGC | MC | N/A | D. rerio | Dnd-KD | Blastula | 20.7 at prim-5 St | N/A | N/A | N/A | Con: 74.1 Dnd-KD: 59.4 TP: 66.7 |
N/A | [8] | |
| M. anguillicaudatus | WT | 10–15 somite St | 1 PGC | MC | N/A | D. rerio | Dnd-KD | Blastula | N/A | N/A | Y at 10–12 mo | N | N/A | N/A | [16] |
| WT | 10–15 somite St | 1 PGC | MC | N/A | D. rerio | Dnd-KD | Blastula | 43.1 at 2 d | N/A | N/A | N/A | 85.7 at 2 d | N/A | [13] | |
| O. masou | WT | 40 d | 5–10 PGCs | MC | N/A | O. mykiss | WT | Newly hatched | 24.4 at 10 dpt | N/A | N/A | N/A | N/A | N/A | [66] |
| O. mykiss | TG:pvasa-GFP | 35 d | 5–10 PGCs | MC | N/A | O. mykiss | WT | 2.5 d | 0 at 30 dpt | N/A | N/A | N/A | 26 at 30 dpt | ♂: 3.9 ♀: 4.2–6.1 |
[15] |
| 35 d | 6 d | 0 at 30 dpt | N/A | N/A | N/A | 62 at 30 dpt | |||||||||
| 35 d | 35 d | 21.6 at 30 dpt | 15.4 at 1–2 y | 16.7 at 1 y | 14.3 at 2 y | 94 at 30 dpt | |||||||||
| 35 d | 35 d | 10–20 * at 30 dpt | N/A | N/A | N/A | N/A | |||||||||
| 40 d | 35 d | 10–20 * at 30 dpt | N/A | N/A | N/A | N/A | |||||||||
| 45 d | 35 d | 0–10 * at 30 dpt | N/A | N/A | N/A | N/A | |||||||||
| 35 d | 40 d | 10–20 * at 30 dpt | N/A | N/A | N/A | N/A | |||||||||
| 40 d | 40 d | 0–10 * at 30 dpt | N/A | N/A | N/A | N/A | |||||||||
| 45 d | 40 d | 0 * at 30 dpt | N/A | N/A | N/A | N/A | |||||||||
| 35 d | 45 d | 0 * at 30 dpt | N/A | N/A | N/A | N/A | |||||||||
| 40 d | 45 d | 0 * at 30 dpt | N/A | N/A | N/A | N/A | |||||||||
| 45 d | 45 d | 0 * at 30 dpt | N/A | N/A | N/A | N/A | |||||||||
| TG:pvasa-GFP | 35 d | 20 PGCs | MC | N/A | O. masou | WT | Newly hatched | 16.7 at 30 dpt | N/A | 13.5 at 1 y | Y at 17 mo | N/A | ♂: 0.4 | [154] | |
| WT | 32 d | 5–10 PGCs | MC | N/A | O. mykiss | WT | Newly hatched | 12.3 at 10 dpt | N/A | N/A | N/A | N/A | N/A | [66] | |
| TG:pvasa-GFP | 30 dpf | 15–20 PGCs | MC | N/A | O. mykiss | WT | 32–34 dpf | Fresh: 12.5 CP (d1): 10.1 at 30 dpt |
Fresh: 10.9 CP (d1): 8.3 at 3 y |
Fresh: 10.3 CP (d1): 7.8 at 3 y |
Fresh: 12.1 CP (d1): 9.1 at 3 y |
Fresh: 98.5 CP (d1): 86.5 at 30 dpt |
Fresh ♂: 0.2–16.3 Fresh ♀: 0.2–7.6 CP (d1) ♂: 2.0–13.5 CP (d1) ♀: 0.1–3.3 |
[53] | |
| S. trutta | WT | 40 d | 5–10 PGCs | MC | N/A | O. mykiss | WT | Newly hatched | 9.4 at 10 dpt | N/A | N/A | N/A | N/A | N/A | [66] |
| Hybrid |
H. huso × A. ruthenus |
Late-neural St | 1 PGC | MC | N/A | C. auratus | WT | Blastula | 9.1 at 3 d | N/A | N/A | N/A | 95.7 at 3 d | N/A | [162] |
Asterisk: estimated average value from the related graphs (no numerics presented), Con: control, CP: cryopreserved, Dnd: dead end, dpt: day post-transplantation, FACS: fluorescence-activated cell sorting, KD: knockdown, MC: manually collected under a fluorescence microscope, N: no (failed, no numerical data presented), N/A: not available, PGC: primordial germ cell, SM: sexual maturity, St: stage, TG: transgenic, TP: transplantation, Y: yes (succeeded, no numerical data presented), WT: wild-type.