. 2022 Feb 10;12(4):423. doi: 10.3390/ani12040423

Table A3.

Gonadal germ cell transplantation into the peritoneal cavity of larvae in fish.

Donor				Enrichment		Recipient			% of Recipients with Gonadal Incorporation of Donor Cells	% of Recipients Producing Donor-Derived Gametes			Survival of Recipients (%)	% of Donor-Derived Offspring	Ref
Species	Fish	Age (Size)	Cells	Method	Purity	Species	Fish	Age (Size)	% of Recipients with Gonadal Incorporation of Donor Cells	Total	Male	Female	Survival of Recipients (%)	% of Donor-Derived Offspring	Ref
A. baerii	WT	4 y	TCs	PDGC	79.4	A. ruthenus	WT	1 wph	60.0 at 90 dpt	N/A	N/A	N/A	Con: 96.7 TP: 95.8	N/A	[39]
	WT	4 y	TCs	N/C	N/A	A. ruthenus	WT	Newly hatched	Fresh: 55.0 CP: 65.0 at 90 dpt	N/A	N/A	N/A	N/A	N/A	[195]
	WT	4 y	OCs	PDGC	70.8	A. ruthenus	WT	1 wph	60.0 at 90 dpt	N/A	N/A	N/A	Con: 96.7 TP: 95.8	N/A	[39]
	WT	4 y	OCs	N/C	N/A	A. ruthenus	WT	Newly hatched	Fresh: 70.0 CP: 55.0 at 90 dpt	N/A	N/A	N/A	N/A	N/A	[195]
A. dabryanus	WT	2 y	50,000 TCs	N/C	N/A	A. dabryanus	WT	7–8 dph	70.0 at 51 dpt	N/A	N/A	N/A	Con: 86.2 TP: 76.7 at 51 dpt	N/A	[196]
A. sinensis	WT	11.5 y	50,000 OCs	N/C	N/A	A. dabryanus	WT	7–8 dph	20.0 at 51 dpt	N/A	N/A	N/A	Con: 86.2 TP: 68.6 at 51 dpt	N/A	[196]
C. carpio	WT	2 y	30,000–50,000 TCs	PDGC	N/A	C. auratus	Dnd-KD	7 d	62.5 at 1 mo	43.7 at 3 y	32.4 at 3 y	11.2 at 3 y	Con: 74 Dnd-KD: 79 Dnd-KD + TP: 72 at 18 mpf	100	[29]
D. rerio	TG:ppiwil1-DsRed	2–3 mo	10–60 SG	PDGC	N/A	D. rerio	Dnd-KD	2 w	N/A	N/A	Cult (3 w): 19 Cult (6 w): 18 at SM	N/A	Cult (3 w): 72.2 Cult (6 w): 64.9 at SM	100	[126]
	TG:pvasa-EGFP	Adult	3000 GFP+ TCs	N/C	N/A	D. rerio	WT	9–10 d	6.7 at 50 dpt	N/A	6.7 at SM	N/A	N/A	3.9–7.3	[101]
	TG:pvasa-EGFP	Adult	3000 GFP+ TCs	N/C	N/A	D. rerio	Dnd-KO	9–10 d	5.0 at 50 dpt	N/A	5.0 at SM	N/A	N/A	100	[101]
	TG:pvasa-EGFP	2 mo	3000–5000 TCs	N/C	N/A	D. rerio	3N	7 d	53.2 at adult	N/A	43.5 at adult	N/A	2N Con: 83.3 3N Con: 75.6 3N TP: 68.9 at adult	100	[145]
	TG:pvasa-EGFP or TG:pβact-YFP	3–6 mo	3000 GFP+ TCs	N/C	N/A	D. rerio	WT	7 d	Fresh: 31.1 CP: 24.4 VF: 22.2 at 50 dpt	N/A	N/A	N/A	Contro: 80 TP: 85 at 50 dpt	N/A	[135]
	TG:pvasa-EGFP or TG:pβact-YFP	3–6 mo	3000 GFP+ TCs	N/C	N/A	D. rerio	Dnd-KD	7 d	Fresh:58.3 CP: 47.4 VF: 50.0 at 50 dpt	N/A	43 at 6 mo	N/A	N/A	100	[135]
	TG:pvasa-DsRed2- vasa;pβact-EGFP	3 mo	OCs	PDGC	N/A	Hybrid	♂ D. albolineatus × ♀ D. rerio	2 w	20.0 at 8 w	17.9 at 6 mo	17.9 at 6 mo	0 at 6 mo	52.7 at 6 mo	100	[69]
	TG:pziwi-Neo;pziwi-DsRed	10–12 w	20–40 FGSCs	PDGC	N/A	D. rerio	Dnd-KD	2 w	N/A	N/A	Cult (3 w): 20.0 Cult (6 w): 16.0 at SM	N/A	Cult (3 w): 73.5 Cult (6 w): 71.7 at SM	100	[123]
	TG:pvasa-EGFP	2 mo	500 OCs	N/C	N/A	D. rerio	3N	7 d	43.6 at adult	N/A	34.5 at adult	N/A	2N Con: 83.3 3N Con: 75.6 3N TP: 61.1 at adult	100	[145]
N. mitsukurii	WT	3 mo	10,000 TCs	N/C	N/A	S. japonicus	WT	5 d	33.5 at 21 dpt	N/A	N/A	N/A	20.3 at 21 dpt	N/A	[99]
								7 d	70.0 at 21 dpt	N/A	N/A	N/A	20.6 at 21 dpt	N/A
								9 d	4.4 at 21 dpt	N/A	N/A	N/A	27.6 at 21 dpt	N/A
	WT	3 mo	3000 TCs	FACS	Unsort: 18.8 Sort: 94.0	N. mitsukurii	WT	12 d	Unsort: 7.6 Sort: 60.0 at 20 dpt	N/A	N/A	N/A	N/A	N/A	[19]
	TG:pHSC-GFP	3 mo	10,000 TCs	N/C	N/A	N. mitsukurii	3N (<100% incidence)	12 dph	2N: 63.3 3N: 56.0 at 18 dpt	2N: 6.1 3N: 31.4 at 6 mo	2N: 5.3 3N: 36.8 at 6 mo	2N: 6.8 3N: 28.9 at 6 mo	41.1 at 18 dpt	2N ♂: 6.8–89.2 3N ♂: 100 2N ♀: 2.2–25.0 3N ♀: 100	[63]
	TG:pHSC-GFP	3 mo	10,000 TCs	N/C	N/A	N. mitsukurii	WT	12 d	58.3 at 2 wpt	N/A	N/A	N/A	68.1 at 2 wpt	N/A	[27]
	TG:pHSC-GFP	3 mo	10,000 TCs	N/C	N/A	Hybrid	♂ P. argentata × ♀ N. mitsukurii, 3N	12 d	58.6 at 2 wpt	34.4 at 6 mo	34.4 at 6 mo	0 at 6 mo	34.7 at 2 wpt	♂: 100	[27]
O. masou	WT	12–13 mo	3000 TCs	FACS	Unsort: 16.4 Sort: 75.6	O. mykiss	WT	Newly hatched	Unsort: 0–25 * Sort: 80.0 at 20 dpt	N/A	N/A	N/A	N/A	N/A	[19]
	WT, black	8 mo	50,000 TCs	N/C	N/A	O. masou	3N	40–42 d	Fresh: 50–60 * CP: 58.2 at 50 dpt	CP: 39.1 at 2 y	CP: 43.5 at 2 y	CP: 34.8 at 2 y	N/A	100	[28]
	WT, black	8 mo	50,000 OCs	N/C	N/A	O. masou	3N	40–42 d	Fresh: 40–60 * CP: 41.2 at 50 dpt	CP: 25.8 at 2 y	CP: 29.3 at 2 y	CP: 23.1 at 2 y	N/A	100	[28]
O. mykiss	Albino, TG:pvasa-GFP	9 mo	18,000 TCs (10,000 GFP+ cells)	FACS	N/A	O. mykiss	WT	Newly hatched	♂: 38.8 ♀: 57.8 at 2 mpt	N/A	50 at 1 y	40 at 2 y	N/A	♂: 5.5 (0.2–40.5) ♀: 2.1 (0.1–9.9)	[37]
	TG:pvasa-GFP	Adult	TCs	N/C	N/A	O. masou	3N	Newly hatched	N/A	N/A	34.5 at 2 y	50.0 at 17 mo; 10.0 at 2–3 y	N/A	♂: 100 ♀: 100	[146]
	TG:pvasa-GFP	8–10 mo	10,000 TCs	DP	95–99	O. mykiss	WT	Newly hatched	Sort: 77.7 Sort + Cult (1 mo): 52.2 at 30 dpt	N/A	N/A	N/A	N/A	N/A	[75]
	TG:pvasa-GFP	16 mo	3000 TCs	FACS	N/A	O. mykiss	WT	Newly hatched	Sort-A: 51.0 Sort-B: 0 at 60 dpt	N/A	N/A	N/A	N/A	N/A	[67]
	TG:pvasa-GFP	7 mo	3000 ASG	N/C	N/A	O. mykiss	WT	Newly hatched	53.6 at 60 dpt	N/A	N/A	N/A	N/A	N/A	[67]
	WT	8–12 mo	3000 TCs	FACS	N/A	O. mykiss	WT	Newly hatched	Unsort: 23.0 Sort: 78.3 at 20 dpt	N/A	N/A	N/A	N/A	N/A	[19]
	TG:pvasa-GFP	8–12 mo			Unsort: 35.9 Sort: 93.2				Unsort: 29.0 Sort: 82.0 at 20 dpt	N/A	N/A	N/A	N/A	N/A
	TG:pvasa-GFP	16 or 24 mo			Sort: 90.0–96.2				N/A	N/A	N/A	N/A	N/A	N/A
	TG:pvasa-GFP	11–12 mo	5000 TCs	DP	>90	O. mykiss	WT	25–27 d	Fresh: 59.7 Cult + Sort: 94.5 Cult + Sort + Tryp-2 h: 43.9 at 20 dpt	N/A	N/A	N/A	N/A	N/A	[73]
	TG:pMlc2-GFP	6 mo	10,000 ASG	PDGC + CE	>90	O. mykiss	WT	Peri-hatching	90 at 6 mo	N/A	90 at 1–2 y	N/A	N/A	1–56	[23]
		1 y			>90				78 at 6 mo	N/A	N/A	N/A	N/A	N/A
		Mature			>70				42 at 6 mo	N/A	N/A	N/A	N/A	N/A
	TG:pvasa-GFP	11 mo	500 GFP+ TCs	N/C	N/A	O. mykiss	3N	32 d	Fresh: 30–40 * CP (F371): 30–40 * at 50 dpt	Fresh: 32.4 CP (F371): 28.6 at 2 y	Fresh: 35.0 CP (F371): 40.0 at 2 y	Fresh: 29.4 CP (F371): 15.4 at 2 y	N/A	100	[61]
		18 mo				O. mykiss		32 d	Fresh: 40–60 * CP (F371): 40–60 * at 30 dpt	N/A	N/A	N/A	N/A	N/A
		11 mo				O. masou		37 d	Fresh: 20–30 * CP (F371): 20–30 * at 50 dpt	Fresh: 27.6 CP (F371): 24.0 at 2 y	Fresh: 33.3 CP (F371): 30.8 at 2 y	Fresh: 23.5 CP (F371): 16.7 at 2 y	N/A	100
	TG:pvasa-GFP	8 mo	30,000 TCs	N/C	N/A	O. masou	WT	38–40 d	63.2 at 20 dpt	N/A	N/A	N/A	N/A	N/A	[124]
	TG:pvasa-GFP	8 mo	30,000 TCs	N/C	N/A	O. masou	Dnd-KD	38–40 d	70 at 20 dpt	54.5 at 2 y	46.7 at 2 y	71.4 at 2 y	N/A	♂: 100 (6 Inds); 72.3 (1 Ind) ♀: 100 (2 Inds); 21.1 (3 Inds)	[124]
	TG:pvasa-GFP	9–11 mo	1000 TCs	FACS (AB No. 80 or No. 95)	Unsort: <20 * Sort (No. 80): 70.7 Sort (No. 95): 80.9	O. mykiss	WT	Newly hatched	Unsort: 1.1 Sort (No. 80): 19.2 Sort (No. 95): 18.4 at 20 dpt	N/A	N/A	N/A	N/A	N/A	[20]
	TG:pvasa-GFP	13–14 mo	<2000 TCs	MACS (AB No. 172)	Unsort: 49.8 Sort: 81.7	O. mykiss	WT	Newly hatched	Unsort: 10–20 * Sort: 30–40 * at 20 dpt	N/A	N/A	N/A	N/A	N/A	[62]
	TG:pvasa-GFP	12–15 mo	15,000 SG	DP	N/A	O. mykiss	3N	30 d	Fresh: 60–80 * Cult: 60–80 * at 20 dpt	Fresh: 89.2 Cult: 65.0 at 1 y	Fresh: 88.0 Cult: 77.6 at 1 y	Fresh: 73.3 Cult: 36.8 at 2 y	N/A	♂: 100 ♀: 100	[147]
	TG:pvasa-GFP	6–9 mo	15,000 OCs	N/C	N/A	O. mykiss	3N	25 d	♂: 50.0 ♀: 47.1 at 5 mo	10.0 at 2 y	15.0 at 2 y	5.0 at 2 y	N/A	♂: 100 ♀: 100	[21]
	TG:pvasa-GFP	9 mo	<10,000 GPF+ OCs	N/C	N/A	O. mykiss	3N	33 d	Fresh: 60–80 * CP: 57.1 at 50 dpt	Fresh: 25.0 CP: 24.0 at 2.5 y	Fresh: 30.8 CP: 28.0 at 2.5 y	Fresh: 18.2 CP: 20.0 at 2.5 y	N/A	Fresh: 100 CP: 100	[56]
	TG:pvasa-GFP	12–14 mo	<10,000 OCs	MACS (AB No. 172)	Unsort: 5.2 Sort: 54.8	O. mykiss	WT	Newly hatched	Unsort: 0–20 * Sort: 60–80 * at 20 dpt	N/A	N/A	N/A	N/A	N/A	[62]
O. nerka	WT	15 mo	<5000 TCs	MACS (AB No. 172)	Unsort: 16.4 Sort: 68.6	O. mykiss	WT	Newly hatched	Unsort: 0–20 * Sort: 40–60 * at 20 dpt	N/A	N/A	N/A	N/A	N/A	[62]
O. niloticus	TG:pβact-EGFP	5–12 mo	20,000 TCs	N/C	N/A	O. niloticus	WT	6 d	0 at 5 mo	N/A	N/A	N/A	N/A	N/A	[137]
								7 d	6.7 at 5 mo	37.5 at 1 y	40.0 at 1 y	33.3 at 1 y	N/A	2.4
								8 d	0 at 5 mo	N/A	N/A	N/A	N/A	N/A
O. latipes	TG:pvasa-GFP	2–4 mo	15,000 TCs	N/C	N/A	O. latipes	WT	7 d	>50 * at 1 mpt	N/A	N/A	N/A	20.0 at 1 mpt	N/A	[55]
			15,000 TCs				WT	11 d	>50 * at 1 mpt	N/A	N/A	N/A	90 at 1 mpt	N/A
			15,000 TCs				WT	14 d	<50 * at 1 mpt	N/A	N/A	N/A	90 at 1 mpt	N/A
			15,000 TCs				WT	19 d	0 * at 1 mpt	N/A	N/A	N/A	90 at 1 mpt	N/A
			15,000 TCs				WT	23 d	0 * at 1 mpt	N/A	N/A	N/A	90 at 1 mpt	N/A
			300 TCs				WT	11 d	10 at 1 mpt	N/A	N/A	N/A	N/A	N/A
			1000 TCs				WT	11 d	10 at 1 mpt	N/A	N/A	N/A	N/A	N/A
			3000 TCs				WT	11 d	60 at 1 mpt	N/A	N/A	N/A	N/A	N/A
			10,000 TCs				WT	11 d	60 at 1 mpt	N/A	N/A	N/A	N/A	N/A
			30,000 TCs				WT	11 d	60 at 1 mpt	N/A	N/A	N/A	N/A	N/A
			15,000 TCs				3N	11 d	N/A	Fresh: 63.6 CP: 70.0 at 2–3 mo	N/A	N/A	N/A	100
	Wnt4b (-/-) mutant	-	15,000 TCs	N/C	N/A	O. latipes	3N	11 d	N/A	CP: 83.3 at 2–3 mo	N/A	N/A	N/A	100	[55]
	Tokyo-medaka (endangered)	1 y					TG:pvasa-GFP, 3N		N/A	CP: 63.9 at 2–3 mo	N/A	N/A	N/A	100
	Kaga (inbred)	-					TG:pvasa-GFP, 3N		N/A	CP: 58.6 at 2–3 mo	N/A	N/A	N/A	100
	Fshr mutant (-/-), XX male	3 mo	3000–9000 TCs	N/C	N/A	Hybrid	♂ O. curvinotus × ♀ O. latipes or ♂ O. latipes × ♀ O. curvinotus	Newly hatched	N/A	N/A	N/A	35.3 at 3 mo	N/A	100	[144]
	TG:pvasa-GFP	2 mo	12,000 TCs	N/C	N/A	Hybrid		Newly hatched	N/A	N/A	N/A	48.3 at 3 mo	N/A	N/A	[144]
	WT	Adult	1000 OCs	PDGC + DP	N/A	O. latipes	WT	11 d	[Cult (10 d)] Con: 15.0 On pDA: 25.0 On pDA/PLL: 0 at 20 d	N/A	N/A	N/A	50.0–80.0 at 20 d	N/A	[197]
	WT	Adult	1000 OCs	PDGC + DP	N/A	O. latipes	WT	11 d	Con: 14.3 Sort: 32.1 at 20 d	N/A	N/A	N/A	57.1–72.4 at 20 d	N/A	[70]
P. dentatus	WT	N/A	TCs	PDGC	N/A	P. olivaceus	3N	17 dph	100 at 14 dpt	N/A	Merged: 100 at 2 y	N/A	[Merged] Con: 27.1 at 3 y TP: 4.0 at 2 y	Merged: Y	[148]
P. dentatus	WT	N/A	TCs	PDGC	N/A	P. olivaceus	3N	20 dph	90.0 at 14 dpt	N/A	Merged: 100 at 2 y	N/A	[Merged] Con: 27.1 at 3 y TP: 4.0 at 2 y	Merged: Y	[148]
P. olivaceus	WT	N/A	TCs	PDGC	N/A	P. olivaceus	3N	16 dph	100 at 14 dpt	N/A	Merged: 100 at 2 y	N/A	[Merged] Con: 27.1 at 3 y TP: 6.8 at 2 y	Merged: Y	[148]
								19 dph	100 at 14 dpt	N/A		N/A
								22 dph	100 at 14 dpt	N/A		N/A
P. spathula	WT	2.5 y	50,000 TCs	PDGC	N/A	A. dabryanus	WT	7–8 dph	80.6 at 2 mo	N/A	N/A	N/A	Con: 76.5 TP: 68.5 at 2 mo	N/A	[198]
S. salar	WT	1 y	2000–5000 TCs	N/C	N/A	O. mykiss	3N (Shasta/ Shasta/albino)	Newly hatched	61.1 at 4 wpt	11.0 at 1–2 y	10.0 at 1–2 y	12.1 at 2 y	N/A	♂: 100 ♀: N/A	[150]
S. trutta	WT	9–12 mo	4000–5000 TCs	FACS (AB No. 95)	Unsort: 21.2 Sort: 80.6	O. mykiss	WT	Newly hatched	Con: 15.8 Sort: 34.7 at 20 dpt	N/A	N/A	N/A	N/A	N/A	[86]
S. leucomaenis	WT	12–13 mo	3000 TCs	FACS	Unsort: 10.1 Sort: 79.9	O. mykiss	WT	Newly hatched	Unsort: 0 Sort: 48.8 at 20 dpt	N/A	N/A	N/A	N/A	N/A	[19]
S. maximus	WT	N/A	TCs	PDGC	N/A	P. olivaceus	3N	15 dph	60.0 at 14 dpt	N/A	Merged: 40.0 at 2 y	N/A	[Merged] Con: 27.1 at 3 y TP: 10.0 at 2 y	Merged: Y	[148]
								18 dph	40.0 at 14 dpt	N/A		N/A
								21 dph	30.0 at 14 dpt	N/A		N/A
S. quinqueradiata	WT	10 mo	20,000 TCs	N/C	N/A	S. quinqueradiata	WT	6 dph	100 at 28 dpt	N/A	N/A	N/A	8.0 at 28 dpt	N/A	[155]
								8 dph	100 at 28 dpt	N/A	N/A	N/A	10.9 at 28 dpt	N/A
								10 dph	85.7 at 28 dpt	N/A	N/A	N/A	15.9 at 28 dpt	N/A
								12 dph	85.7 at 28 dpt	N/A	N/A	N/A	29.6 at 28 dpt	N/A
								Merged	N/A	N/A	100 at 1.5–2.5 y	100 at 2.5 y	5.08 at SM	♂: 66.6 (19.6–98.8) ♀: 63.2 (17.0–97.5)
T. rubripes	WT	1 y	4000–6000 TCs	N/C	N/A	T. alboplumbeus	3N (<100% incidence)	1 dph	50 at 4 wpt	N/A	38.3 at 11 mo	31.3 at 2 y	47 at 72 wpt	♂: 100 ♀: 5–95	[138]
	WT	1 y	4000–6000 TCs	N/C	N/A	T. alboplumbeus	3N (<100% incidence)	5 dph	33.3 at 4 wpt	N/A	23.7 at 11 mo	23.1 at 2 y	41.4 at 72 wpt	♂: 100 ♀: 5–95	[138]
	WT	10–12 mo	5000 TCs	N/C	N/A	T. alboplumbeus	3N (<100% incidence)	1 dph	Fresh: 39.1 CP: 38.9 at 40 dph	N/A	[CP] 2N: 36.4 3N: 64.2 at 10 mph	[CP] 2N: 0 3N: 1.6 at 2 y	Con: 41.4 Fresh: 21.8 CP: 14.5 at 40 dph	2N ♂: 3.2–87.5 (3 Inds) 2N ♀: 0 (10 Inds) 3N ♂: 100 (11 Inds); 62.5 (1 Ind) 3N ♀: 100 (1 Ind)	[120]
	WT	1 y	5000 TCs	N/C	N/A	T. alboplumbeus	WT	1 dph	40.0 at 40 dph	N/A	N/A	N/A	Con: 23.8 at 40 dph; 45.3 at 12 mph TP: 14.8 at 40 dph	N/A	[127]
	WT	1 y	5000 TCs	N/C	N/A	T. alboplumbeus	Dnd-KD	1 dph	40.5 at 40 dph	N/A	91.7 at 10 mo	26.7 at 2 y	Dnd-KD: 20.3 and 35.0 Dnd-KD + TP: 15.8 and 16.9 at 40 dph and 12 mph	♂: 100 (7 Inds); 12.5–87.5 (2 Inds) ♀: 100 (3 Inds)	[127]
T. maccoyii	WT	57.0 kg	TCs	PDGC	N/A	S. lalandi	WT	6–7 dph	33.3 at 18 dpt	N/A	N/A	N/A	9.1 at 64 dph	N/A	[47]
T. maccoyii	WT	57.0 kg	TCs	PDGC	N/A	S. lalandi	WT	9–10 dph	37.5 at 18 dpt	N/A	N/A	N/A	9.2 at 64 dph	N/A	[47]
T. orientalis	WT	0 y	TCs	N/C	N/A	S. japonicus	WT	7 d	0 at 21 dpt	N/A	N/A	N/A	N/A	N/A	[22]
		1 y							5.4 at 21 dpt	N/A	N/A	N/A	N/A	N/A
		3 y							0 at 21 dpt	N/A	N/A	N/A	N/A	N/A
	WT	2–4 y	>10,000 TCs	FACS (AB No. 152)	Unsort: 16.6 Sort: 77.3	N. mitsukurii	WT	12 dph	PDGC Sort (PKH26 labled): 33.3 PDGC Sort (No. 152 AB labeled): 63.3 at 14 dpt	N/A	N/A	N/A	N/A	N/A	[45]
	WT	3 y	>10,000 TCs	PDGC	N/A	Hybrid	♂ S. japonicus × ♀ S. australasicus	10 d	100 at 14 dpt	N/A	N/A	N/A	N/A	N/A	[48]
	WT	2 y	>30,000 TCs	N/C	N/A	S. japonicus	WT	7 dph	Y at 14 dpt and 4 mpt	N/A	N/A	N/A	N/A	N/A	[46]
	WT	2 y	>30,000 TCs	N/C	N/A	E. affinis	WT	10 dph	Y at 14 dpt and 3 mpt	N/A	N/A	N/A	N/A	N/A	[46]

2N: diploid, 3N: triploid, AB: antibody, ASG: type A spermatogonia, Asterisk: estimated average value from the related graphs (no numerics presented), CE: centrifugal elutriation, Con: control, CP: cryopreserved, Cult: cultured, Dnd: dead end, DP: differential plating, dph: day post-hatching, dpt: day post-transplantation, FACS: fluorescence-activated cell sorting, FGSC: female germline stem cell, Ind: individual, KD: knockdown, KO: knockout, MACS: magnetic-activated cell sorting, mpt: month post-transplantation, N/A: not available, N/C: not carried out, OC: ovarian cell, PDGC: Percoll density gradient centrifugation, SG: spermatogonia, SM: sexual maturity, Sort: sorted, TC: testicular cell, TG: transgenic, TP: transplantation, Trip: trypsinized, Unsort: unsorted, VF: vitrified, wph: week post-hatching, wpt: week post-transplantation, WT: wild-type, Y: yes (succeed, no numerical data presented).