. 2022 Feb 14;11(2):385. doi: 10.3390/antiox11020385

Table 2.

The antioxidant activities of different solvent extracts from Anemarrhena asphodeloides determined with DPPH, ABTS, superoxide radical scavenging, and ferric-reducing antioxidant power (FRAP) assays.

Extracting Solvents	DPPH IC₅₀ (μg/mL)		ABTS IC₅₀ (μg/mL)		Superoxide IC₅₀ (μg/mL)		FRAP (mM/g) ^c (TE)
Extracting Solvents	AA	Salt-Fried AA	AA	Salt-Fried AA	AA	Salt-Fried AA	AA	Salt-Fried AA
n-Hexane	>400	>400	305.8 ± 10.4 *	>400	>400	>400	95.9 ± 5.4 **	42.6 ± 5.7 *
Chloroform	>400	>400	73.8 ± 2.3 **	64.3 ± 3.9 **	>400	>400	278.5 ± 7.2 ***	346.3 ± 5.3 ***
Dichloromethane	>400	>400	95.4 ± 5.5 **	40.9 ± 2.5 **	>400	>400	264.5 ± 14.4 **	355.6 ± 5.1 ***
Ethyl acetate	241.2 ± 15.4 *	219.4 ± 6.4 *	75.3 ± 3.7 **	24.1 ± 1.2 **	>400	>400	282.7 ± 7.3 ***	485.5 ± 4.3 ***
Acetone	123.6 ± 3.9 *	169.8 ± 8.6 *	48.5 ± 2.9 **	41.9 ± 0.7 **	>400	>400	381.5 ± 8.3 ***	481.6 ± 19.0 **
Methanol	193.3 ± 9.6 *	181.2 ± 5.2 *	216.0 ± 3.9 *	119.4 ± 4.2 *	>400	>400	152.2 ± 12.8 **	255.2 ± 4.6 ***
Ethanol	150.1 ± 1.1 *	194.2 ± 4.3 *	177.8 ± 3.4 *	112.4 ± 3.6 *	>400	>400	203.2 ± 12.4 **	296.0 ± 11.7 **
Water	127.7 ± 2.3 *	113.9 ± 8.6 *	117.9 ± 0.6 *	97.5 ± 1.6 *	369.9 ± 4.3 *	295.4 ± 6.1 *	230.0 ± 13.8 **	323.7 ± 14.9 **
BHT ^a	35.5 ± 0.6 **	39.7 ± 1.4 **	20.6 ± 0.2 **	19.6 ± 0.4 **	N.A. ^b	N.A. ^b	4297.0 ± 48.4 ***	4216.1 ± 66.5 ***

Results are expressed as half inhibitory concentration (IC₅₀) of each free-radical scavenging activity. ^a Butylated hydroxytoluene (BHT) used as positive control. ^b N.A. indicates not available (poor solubility). ^c Ferric-reducing antioxidant power (FRAP) assay was expressed as millimolar (mM) of Trolox equivalents (TE) per gram of extract. * p < 0.05; ** p < 0.01; *** p < 0.001 compared with the control. AA means Anemarrhena asphodeloides. Salt-fried AA means salt-fried Anemarrhena asphodeloides.