Table 5.
Identification and quantification of the main active compounds of A. asphodeloides in different solvent extracts.
| Extracting Solvents |
Neomangiferin (mg/kg) |
Timosaponin A-III (mg/kg) |
Isomangiferin (mg/kg) |
Mangiferin (mg/kg) |
Total Amount (mg/kg) |
|---|---|---|---|---|---|
| Water (AA) | 2.5 ± 0.5 * | 5.4 ± 0.7 ** | 2.8 ± 0.4 ** | 4.4 ± 0.6 ** | 15.1 ± 0.6 *** |
| Methanol (AA) | 2.0 ± 0.4 * | 3.6 ± 0.4 ** | 3.3 ± 0.4 ** | 3.8 ± 0.4 ** | 12.8 ± 1.6 ** |
| Ethanol (AA) | 1.6 ± 0.7 | 3.1 ± 0.4 ** | 1.0 ± 0.4 * | 3.8 ± 0.2 *** | 9.6 ± 0.5 *** |
| Acetone (AA) | 7.9 ± 1.1 ** | 4.8 ± 1.3 * | 9.6 ± 1.2 * | 6.5 ± 1.9 * | 28.8 ± 1.2 *** |
| Ethyl acetate (AA) | 6.2 ± 0.9 ** | 2.4 ± 0.9 * | 2.2 ± 0.8 * | 4.2 ± 1.0 * | 15.1 ± 0.7 *** |
| Chloroform (AA) | 4.4 ± 1.5 * | 13.9 ± 1.2 ** | 23.7 ± 1.9 ** | 5.5 ± 1.2 * | 47.5 ± 1.5 *** |
| Dichloromethane (AA) | 8.7 ± 1.4 ** | 4.3 ± 1.4 * | 3.3 ± 0.9 * | 7.2 ± 1.3 * | 23.5 ± 1.3 ** |
| n-Hexane (AA) | 2.7 ± 1.2 | 21.3 ± 1.1 *** | 12.3 ± 1.9 ** | 7.6 ± 1.2 ** | 43.9 ± 1.4 *** |
| Water (salt-fried AA) | 0.9 ± 0.5 | 3.4 ± 0.8 * | 3.8 ± 0.6 ** | 4.4 ± 1.1 * | 12.6 ± 0.8 ** |
| Methanol (salt-fried AA) | 3.0 ± 0.7 * | 5.6 ± 0.9 ** | 5.3 ± 0.8 ** | 7.8 ± 0.7 ** | 21.8 ± 0.8 *** |
| Ethanol (salt-fried AA) | 1.9 ± 0.9 | 3.1 ± 0.9 * | 3.0 ± 0.8 * | 5.8 ± 1.2 * | 13.8 ± 1.0 ** |
| Acetone (salt-fried AA) | 2.9 ± 1.0 * | 3.8 ± 1.2 * | 9.6 ± 1.3 ** | 5.5 ± 1.2 * | 21.8 ± 1.2 ** |
| Ethyl acetate (salt-fried AA) | 3.2 ± 0.9 * | 9.6 ± 1.3 ** | 4.2 ± 1.6 * | 10.2 ± 1.2 ** | 27.3 ± 1.3 *** |
| Chloroform (salt-fried AA) | 3.4 ± 1.2 * | 12.9 ± 1.4 ** | 23.7 ± 1.7 ** | 6.5 ± 1.2 * | 46.5 ± 1.4 *** |
| Dichloromethane (salt-fried AA) | 2.7 ± 1.6 | 10.3 ± 1.4 ** | 3.6 ± 1.2 * | 6.2 ± 1.6 * | 22.8 ± 1.5 ** |
| n-Hexane (salt-fried AA) | 12.7 ± 1.2 ** | 3.3 ± 1.1 * | 21.3 ± 1.9 ** | 4.6 ± 1.2 * | 41.9 ± 1.4 *** |
Results are expressed as milligrams of each compound in kilogram of extract. * p < 0.05; ** p < 0.01; *** p < 0.001 compared with the blank. AA means Anemarrhena asphodeloides. Salt-fried AA means salt-fried Anemarrhena asphodeloides.