Table 6.
The antioxidant activities of isolated components from Anemarrhena asphodeloides determined with DPPH, ABTS, superoxide radical scavenging, and ferric-reducing antioxidant power (FRAP) assays.
| Compounds | DPPH IC50 (μg/mL) |
ABTS IC50 (μg/mL) |
Superoxide IC50 (μg/mL) |
FRAP (mM/g) c (TE) |
|---|---|---|---|---|
| Mangiferin | 5.4 ± 0.3 * | 3.7 ± 0.2 * | 53.8 ± 2.4 * | 9371.4 ± 183.9 *** |
| Timosaponin A-III | >200 | >200 | >200 | 19.5 ± 3.9 * |
| Neomangiferin | >200 | 81.9 ± 4.6 ** | >200 | 83.1 ± 5.3 ** |
| Isomangiferin | 16.7 ± 1.1 * | 5.6 ± 0.4 * | 155.3 ± 13.8 * | 6359.9 ± 176.8 *** |
| BHT a | 27.2 ± 1.9 * | 15.4 ± 0.9 * | N.A. b | 3963.9 ± 104.0 *** |
Results are expressed as half inhibitory concentration (IC50) of each free-radical scavenging activity. a Butylated hydroxytoluene (BHT) used as positive control. b N.A. indicates not available (poor solubility). c Ferric-reducing antioxidant power (FRAP) assay expressed as millimolar (mM) of Trolox equivalents (TE) per gram of extract. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with the control.