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. 2022 Jan 28;11(2):210. doi: 10.3390/biology11020210

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Scheme of the procedures carried out during the study. (A) The collection of the semen was performed according to the fractions of the ejaculate (F1 = rich fraction; F2 = F1 + intermediate fraction; F3 = F2 + poor fraction). The seminal doses were prepared for each type of ejaculate and kept at refrigeration until use. (B) On day 3 of conservation, the seminal doses were used for controlling the semen quality and for AI. The inseminated sows were diagnosed in pregnancy by ultrasound, and offspring was evaluated (growth and blood analysis). (C) Seminal doses were conserved in a temperature-controlled refrigerator during the whole period of the trial (March and April 2021). Images (A,B) were created on Biorender.com (accessed on 1 March 2021).