Table 2.
Spermatozoa quality parameters from different accumulative ejaculate fractions (F1, F2, F3) analyzed after 3 days of storage at ~16 °C. Data are expressed as the mean ± SEM (standard error of the mean).
| F1 (n = 17) |
F2 (n = 17) |
F3 (n = 17) |
p-Value | |
|---|---|---|---|---|
| Total motility (%) | 90.89 ± 0.92 | 90.32 ± 0.68 | 89.11 ± 0.79 | 0.65 |
| Progressive motility (%) | 37.53 ± 1.27 | 35.42 ± 1.89 | 39.16 ± 1.41 | 0.61 |
| VCL (µm/s) | 61.68 ± 2.72 | 59.26 ± 2.65 | 55.00 ± 1.54 | 0.50 |
| VSL (µm/s) | 22.32 ± 0.84 | 21.53 ± 0.85 | 23.00 ± 0.73 | 0.76 |
| VAP (µm/s) | 39.32 ± 1.50 | 38.32 ± 1.06 | 37.53 ± 0.93 | 0.82 |
| ALH (µm) | 1.95 ± 0.09 | 2.00 ± 0.06 | 1.89 ± 0.04 | 0.82 |
| LIN (%) | 38.26 ± 1.69 | 38.53 ± 1.75 | 42.53 ± 1.34 | 0.48 |
| STR (%) | 58.16 ± 1.62 | 57.00 ± 1.83 | 61.79 ± 1.51 | 0.47 |
| WOB (%) | 64.63 ± 1.25 | 66.32 ± 1.19 | 68.68 ± 0.97 | 0.35 |
| BCF (Hz) | 7.26 ± 0.15 | 6.79 ± 0.14 | 6.79 ± 0.11 | 0.24 |
| Viability (%) | 93.05 ± 0.25 | 92.89 ± 0.30 | 92.11 ± 0.27 | 0.32 |
| Acrosome integrity (%) | 95.16 ± 0.19 | 94.74 ± 0.23 | 94.32 ± 0.21 | 0.27 |
| Mitochondrial activity (%) | 92.37 ± 0.34 | 92.37 ± 0.38 | 91.79 ± 0.34 | 0.74 |
| DNA fragmentation (%) | 0.42 ± 0.09 | 1.11 ± 0.28 | 0.68 ± 0.15 | 0.33 |